Tolhuin is a town in the province of Tierra del Fuego, Argentina. It has 1,382 inhabitants as per the 2001 census [INDEC]. It is located on the eastern shore of Lake Fagnano, in the southern part of the Isla Grande de Tierra del Fuego. It is the third biggest settlement on the Argentine side of Tierra del Fuego after Ushuaia and Río Grande.